FLOW CYTOMETRY-BASED QUANTITATIVE ANALYSIS OF CELLULAR PROTEIN EXPRESSION IN APOPTOSIS SUBPOPULATIONS: A PROTOCOL

Flow cytometry-based quantitative analysis of cellular protein expression in apoptosis subpopulations: A protocol

Flow cytometry techniques utilizing dual staining with annexin V and propidium iodide (PI) provide a robust method for quantitatively analyzing apoptosis induction.Annexin V binds phosphatidylserine exposed on the outer leaflet of the plasma membrane during early apoptosis, while PI permeates late apoptotic/necrotic cells.Simultaneous staining allo

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Localization and flow-dependency on blending techniques

Lagrangian extrapolation nowcasting is still the most accurate technique for predicting precipitation for the first hours despite the improvements achieved by data assimilation in high-resolution numerical weather prediction models.Yet, the combination of both sources by blending techniques is necessary for creating a seamless forecast for the nowc

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Effects of transcranial direct current stimulation on brain activity and cortical functional connectivity in children with autism spectrum disorders

IntroductionTranscranial direct current stimulation (tDCS) has emerged as a therapeutic option to mitigate symptoms in individuals with autism spectrum disorder (ASD).Our study investigated the effects of a two-week regimen of tDCS targeting the left dorsolateral prefrontal cortex (DLPFC) in children with ASD, examining changes in rhythmic brain ac

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