Flow cytometry-based quantitative analysis of cellular protein expression in apoptosis subpopulations: A protocol
Flow cytometry techniques utilizing dual staining with annexin V and propidium iodide (PI) provide a robust method for quantitatively analyzing apoptosis induction.Annexin V binds phosphatidylserine exposed on the outer leaflet of the plasma membrane during early apoptosis, while PI permeates late apoptotic/necrotic cells.Simultaneous staining allo